Comparative in silico design and validation of GPS™ CoVID-19 dtec-RT-qPCR test.

ABSTRACT:

Aims

Providing a ready-to-use reverse transcriptase qPCR (RT-qPCR) method fully validated to detect the SARS-CoV-2 with a higher exclusivity than this shown by early published RT-qPCR designs.

Methods and results

The specificity of the GPS™ CoVID-19 dtec-RT-qPCR test by analysis of sequence alignments was approached and compared with other RT-qPCR designs. The GPS™ CoVID-19 dtec-RT-qPCR test was validated following criteria of UNE/EN ISO 17025:2005 and ISO/IEC 15189:2012. Diagnostic validation was achieved by two independent reference laboratories, the Instituto de Salud Carlos III, (Madrid, Spain), the Public Health England (Colindale, London, UK), and received the label CE-IVD. The GPS design showed the highest exclusivity and passed all parameters of validation with stric

SUBMITTER: Martinez-Murcia A 

PROVIDER: S-EPMC7405274 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature