Arsenite Exposure Displaces Zinc from ZRANB2 Leading to Altered Splicing.

ABSTRACT: Exposure to arsenic, a class I carcinogen, affects 200 million people globally. Skin is the major target organ, but the molecular etiology of arsenic-induced skin carcinogenesis remains unclear. Arsenite (As³⁺)-induced disruption of alternative splicing could be involved, but the mechanism is unknown. Zinc finger proteins play key roles in alternative splicing. As³⁺ can displace zinc (Zn²⁺) from C3H1 and C4 zinc finger motifs (zfm's), affecting protein function. ZRANB2, an alternative splicing regulator with two C4 zfm's integral to its structure and splicing function, was chosen as a candidate for this study. We hypothesized that As³⁺ could displace Zn²⁺ from ZRANB2, altering its structure, expression, and splicing function. As³⁺

SUBMITTER: Banerjee M 

PROVIDER: S-EPMC7405655 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature