ABCA1 Regulates IOP by Modulating Cav1/eNOS/NO Signaling Pathway.

ABSTRACT:

Purpose

This study aimed to investigate the role and pathophysiological mechanism of ATP binding cassette transporter A1 (ABCA1) in regulating the IOP and aqueous humor outflow.

Methods

ABCA1 expression was measured in trabecular meshwork samples obtained from patients with POAG and human donor eyes by Western blot. To further evaluate the functional significance of ABCA1, porcine angular aqueous plexus (AAP) cells, which are equivalent to human Schlemm's canal endothelial cells, were either treated with ABCA1 agonist GW3965 or transduced with lentivirus expressing ABCA1-shRNA. Transendothelial electrical resistance, protein expression, and nitric oxide (NO) concentration were measured. GW3965 was administered by intracameral injection. IOP and aqueous humor outflow facility

SUBMITTER: Hu C 

PROVIDER: S-EPMC7405707 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature