Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials.

ABSTRACT:

Background

Fast and reliable detection of SARS-CoV-2 is crucial for efficient control of the COVID-19 pandemic. Due to the high demand for SARS-CoV-2 testing there is a worldwide shortage of RNA extraction reagents. Therefore, extraction-free RT-qPCR protocols are urgently needed.

Objectives

To establish a rapid RT-qPCR protocol for the detection of SARS-CoV-2 without the need of RNA extraction suitable for all respiratory materials.

Material and methods

Different SARS-CoV-2 positive respiratory materials from our routine laboratory were used as crude material after heat inactivation in direct RT-qPCR with the PrimeDirect™ Probe RT-qPCR Mix (TaKaRa). SARS-CoV-2 was detected using novel primers targeted to the E-gene.

Results

The protocol for the detection of SA

SUBMITTER: Lubke N 

PROVIDER: S-EPMC7405857 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature