In vivo assessment of respiratory burst inhibition by xenobiotic exposure using larval zebrafish.

ABSTRACT: Currently, assessment of the potential immunotoxicity of a given agent involves a tiered approach for hazard identification and mechanistic studies, including observational studies, evaluation of immune function, and measurement of susceptibility to infectious and neoplastic diseases. These studies generally use costly low-throughput mammalian models. Zebrafish, however, offer an excellent alternative due to their rapid development, ease of maintenance, and homology to mammalian immune system function and development. Larval zebrafish also are a convenient model to study the innate immune system with no interference from the adaptive immune system. In this study, a respiratory burst assay (RBA) was utilized to measure reactive oxygen species (ROS) production after developmental xenobiotic

SUBMITTER: Phelps DW 

PROVIDER: S-EPMC7405951 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature