Utility of LysM-cre and Cdh5-cre Driver Mice in Retinal and Brain Research: An Imaging Study Using tdTomato Reporter Mouse.

ABSTRACT:

Purpose

The lysozyme 2 (Lyz2 or LysM) cre mouse is extensively used to achieve genetic manipulation in myeloid cells and it has been widely employed in retinal research. However, LysM has been recently described to be expressed in brain neurons and there is a debate on whether it is also expressed by resident microglia in addition to infiltrating macrophages.

Methods

We examined LysM-cre recombination in retinal tissue using a LysM-cre/tdTomato reporter mouse together with immunolabeling for several retinal cell markers. We further compared LysM-cre tdTomato recombination with that of Cdh5-cre driver, which is expressed in both endothelial and hematopoietic cells.

Results

LysM-cre was strongly expressed in most microglia/resident macrophages in neonatal retinas (P8) a

SUBMITTER: Fouda AY 

PROVIDER: S-EPMC7405957 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature