Distribution of DNA damage in the human sperm nucleus: implications of the architecture of the sperm head.

ABSTRACT: The sperm nucleus is prone to sustain DNA damage before and after ejaculation. Distribution of the damage is not homogeneous, and the factors determining differential sensitivity among nuclear regions have not yet been characterized. Human sperm chromatin contains three structural domains, two of which are considered the most susceptible to DNA damage: the histone bound domain, harboring developmental related genes, and the domain associated with nuclear matrix proteins. Using a quantitative polymerase chain reaction (qPCR) approach, we analyzed the number of lesions in genes homeobox A3 (HOXA3), homeobox B5 (HOXB 5), sex-determining region Y (SRY)-box 2 (SOX2), β-GLOBIN, rDNA 18S, and rDNA 28S in human sperm after ultraviolet irradiation (400 μW cm^-2, 10 min), H₂O

SUBMITTER: Gonzalez-Rojo S 

PROVIDER: S-EPMC7406100 | biostudies-literature | 2020 Jul-Aug

REPOSITORIES: biostudies-literature