Fentanyl vapor self-administration model in mice to study opioid addiction.

ABSTRACT: Intravenous drug self-administration is considered the "gold standard" model to investigate the neurobiology of drug addiction in rodents. However, its use in mice is limited by frequent complications of intravenous catheterization. Given the many advantages of using mice in biomedical research, we developed a noninvasive mouse model of opioid self-administration using vaporized fentanyl. Mice readily self-administered fentanyl vapor, titrated their drug intake, and exhibited addiction-like behaviors, including escalation of drug intake, somatic signs of withdrawal, drug intake despite punishment, and reinstatement of drug seeking. Electrophysiological recordings from ventral tegmental area dopamine neurons showed a lower amplitude of GABA_B receptor-dependent currents during pro

SUBMITTER: Moussawi K 

PROVIDER: S-EPMC7406365 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature