Comparative Characterization of Aspergillus Pectin Lyases by Discriminative Substrate Degradation Profiling.

ABSTRACT: Fungal genomes often contain several copies of genes that encode carbohydrate active enzymes having similar activity. The copies usually have slight sequence variability, and it has been suggested that the multigenecity represents distinct reaction optima versions of the enzyme. Whether the copies represent differences in substrate attack proficiencies of the enzyme have rarely been considered. The genomes of Aspergillus species encode several pectin lyases (EC 4.2.2.10), which all belong to polysaccharide lyase subfamily PL1_4 in the CAZy database. The enzymes differ in terms of sequence identity and phylogeny, and exhibit structural differences near the active site in their homology models. These enzymes catalyze pectin degradation via eliminative cleavage of the α-(1,4) glycosidi

SUBMITTER: Zeuner B 

PROVIDER: S-EPMC7406575 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature