Detection of SARS-CoV-2 by RNAscope^® in situ hybridization and immunohistochemistry techniques.

ABSTRACT: In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope^®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

SUBMITTER: Carossino M 

PROVIDER: S-EPMC7406679 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature