β₂-integrin LFA1 mediates airway damage following neutrophil transepithelial migration during respiratory syncytial virus infection.

ABSTRACT: Respiratory syncytial virus (RSV) bronchiolitis is the most common cause of infant hospital admissions, but there is limited understanding of the mechanisms of disease, and no specific antiviral treatment. Using a novel in vitro primary transepithelial neutrophil migration model and innovative imaging methods, we show that RSV infection of nasal airway epithelium increased neutrophil transepithelial migration and adhesion to infected epithelial cells, which is associated with epithelial cell damage and reduced ciliary beat frequency, but also with a reduction in infectious viral load.Following migration, RSV infection results in greater neutrophil activation, degranulation and release of neutrophil elastase into the airway surface media compared to neutrophils that migrated across m

SUBMITTER: Herbert JA 

PROVIDER: S-EPMC7406857 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature