Integrative Analysis of Proteome and Transcriptome Dynamics during Bacillus subtilis Spore Revival.

ABSTRACT: Bacillus subtilis spores can reactivate their metabolism through germination upon contact with germinants and can develop into vegetative cells upon outgrowth. However, the mechanisms at the basis of the molecular machinery that triggers the spore germination and outgrowth processes are still largely unclear. To gain further insights into these processes, the transcriptome and proteome changes occurring during the conversion of spores to vegetative cells were analyzed in the present study. For each time point sampled, the changes in the spore proteome were quantitatively monitored relative to the proteome of metabolically ¹⁵N-labeled vegetative cells. Of the quantified proteins, 60% are shared by vegetative cells and spores, indicating that the spores have a minimal prote

SUBMITTER: Swarge B 

PROVIDER: S-EPMC7407066 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature