Protein Conformational Changes in Breast Cancer Sera Using Infrared Spectroscopic Analysis.

ABSTRACT: Protein structural alterations, including misfolding and aggregation, are a hallmark of several diseases, including cancer. However, the possible clinical application of protein conformational analysis using infrared spectroscopy to detect cancer-associated structural changes in proteins has not been established yet. The present study investigates the applicability of Fourier transform infrared spectroscopy in distinguishing the sera of healthy individuals and breast cancer patients. The cancer-associated alterations in the protein structure were analyzed by fitting the amide I (1600-1700 cm^-1) band of experimental curves, as well as by comparing the ratio of the absorbance values at the amide II and amide III bands, assigning those as the infrared spectral signatures. The snaps

SUBMITTER: Ghimire H 

PROVIDER: S-EPMC7407230 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature