Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae.

ABSTRACT: Anisakis simplex third-stage larvae are the main source of hidden allergens in marine fish products. Some Anisakis allergens are thermostable and, even highly processed, could cause hypersensitivity reactions. However, Anisakis proteome has not been studied under autoclaving conditions of 121 °C for 60 min, which is an important process in the food industry. The aim of the study was the identification and characterization of allergens, potential allergens, and other proteins of heat-treated A. simplex larvae. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify 470 proteins, including allergens-Ani s 1, Ani s 2, Ani s 3, Ani s 4, Ani s 5-and 13 potential allergens that were mainly homologs of Anisakis spp., Ascaris spp., a

SUBMITTER: Kochanowski M 

PROVIDER: S-EPMC7407331 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature