Inflammation Differentially Modulates the Biological Features of Adult Derived Human Liver Stem/Progenitor Cells.

ABSTRACT: The progression of mesenchymal stem cell-based therapy from concept to cure closely depends on the optimization of conditions that allow a better survival and favor the cells to achieve efficient liver regeneration. We have previously demonstrated that adult-derived human liver stem/progenitor cells (ADHLSC) display significant features that support their clinical development. The current work aims at studying the impact of a sustained pro-inflammatory environment on the principal biological features of ADHLSC in vitro. ADHLSC from passages 4-7 were exposed to a cocktail of inflammatory cytokines for 24 h and 9 days and subsequently analyzed for their viability, expression, and secretion profiles by using flow cytometry, RT-qPCR, and antibody array assay. The impact of inflammation on the

SUBMITTER: El-Kehdy H 

PROVIDER: S-EPMC7408415 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature