Probing Structure and Function of Alkali Sensor IRR with Monoclonal Antibodies.

ABSTRACT: To study the structure and function of the pH-regulated receptor tyrosine kinase insulin receptor-related receptor (IRR), а member of the insulin receptor family, we obtained six mouse monoclonal antibodies against the recombinant IRR ectodomain. These antibodies were characterized in experiments with exogenously expressed full-length IRR by Western blotting, immunoprecipitation, and immunocytochemistry analyses. Utilizing a previously obtained set of IRR/IR chimeras with swapped small structural domains and point amino acid substitutions, we mapped the binding sites of the obtained antibodies in IRR. Five of them showed specific binding to different IRR domains in the extracellular region, while one failed to react with the full-length receptor. Unexpectedly, we found that 4D5 antibody ca

SUBMITTER: Goryashchenko AS 

PROVIDER: S-EPMC7408431 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature