Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa.

ABSTRACT: Current treatments against bacterial infections have severe limitations, mainly due to the emergence of resistance to conventional antibiotics. In the specific case of Pseudomonasaeruginosa strains, they have shown a number of resistance mechanisms to counter most antibiotics. Human secretory RNases from the RNase A superfamily are proteins involved in a wide variety of biological functions, including antimicrobial activity. The objective of this work was to explore the intracellular antimicrobial action of an RNase 3/1 hybrid protein that combines RNase 1 high catalytic and RNase 3 bactericidal activities. To achieve this, we immobilized the RNase 3/1 hybrid on Polyetheramine (PEA)-modified magnetite nanoparticles (MNPs). The obtained nanobioconjugates were tested in macrophage-derived TH

SUBMITTER: Rangel-Munoz N 

PROVIDER: S-EPMC7408537 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature