Identification of 5-Fluoro-5-Deoxy-Ribulose as a Shunt Fluorometabolite in <i>Streptomyces sp</i>. MA37.

ABSTRACT: A fluorometabolite, 5-fluoro-5-deoxy-D-ribulose (5-FDRul), from the culture broth of the soil bacterium Streptomyces sp. MA37, was identified through a combination of genetic manipulation, chemo-enzymatic synthesis and NMR comparison. Although 5-FDRul has been chemically synthesized before, it was not an intermediate or a shunt product in previous studies of fluorometalism in S. cattleya. Our study of MA37 demonstrates that 5-FDRul is a naturally occurring fluorometabolite, rendering it a new addition to this rare collection of natural products. The genetic inactivation of key biosynthetic genes involved in the fluorometabolisms in MA37 resulted in the increased accumulation of unidentified fluorometabolites as observed from ¹⁹F-NMR spectral comparison among the wi

SUBMITTER: Wu L 

PROVIDER: S-EPMC7408626 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature