The RNA Replication Site of Tula Orthohantavirus Resides within a Remodelled Golgi Network.

ABSTRACT: : The family Hantaviridae within the Bunyavirales order comprises tri-segmented negative sense RNA viruses, many of which are rodent-borne emerging pathogens associated with fatal human disease. In contrast, hantavirus infection of corresponding rodent hosts results in inapparent or latent infections, which can be recapitulated in cultured cells that become persistently infected. In this study, we used Tula virus (TULV) to investigate the location of hantavirus replication during early, peak and persistent phases of infection, over a 30-day time course. Using immunofluorescent (IF) microscopy, we showed that the TULV nucleocapsid protein (NP) is distributed within both punctate and filamentous structures, with the latter increasing in size as the infection progresses.

SUBMITTER: Davies KA 

PROVIDER: S-EPMC7408811 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature