Apigenin and Ethaverine Hydrochloride Enhance Retinal Vascular Barrier In Vitro and In Vivo.

ABSTRACT:

Purpose

This study aims to develop an impedance-based drug screening platform that will help identify drugs that can enhance the vascular barrier function by stabilizing vascular endothelial cell junctions.

Methods

Changes in permeability of cultured human retinal microvascular endothelial cells (HRMECs) monolayer were monitored in real-time with the xCELLigence RTCA system. Using this platform, we performed a primary screen of 2100 known drugs and confirmed hits using two additional secondary permeability assays: the transwell permeability assay and the XPerT assay. The cellular and molecular mechanisms of action and in vivo therapeutic efficacy were also assessed.

Results

Eleven compounds blocked interleukin 1 beta (IL-1β) induced hyperpermeability in the primary sc

SUBMITTER: Jiang W 

PROVIDER: S-EPMC7409011 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature