Optimization of a Portable Adenosine Triphosphate Bioluminescence Assay Coupled with a Receiver Operating Characteristic Model to Assess Bioaerosol Concentrations on Site.

ABSTRACT: Rapid monitoring of the microbial content in indoor air is an important issue. In this study, we develop a method for applying a Coriolis sampler coupled with a portable ATP luminometer for characterization of the collection efficiency of bioaerosol samplers and then test this approach in field applications. The biological collection efficiencies of the Coriolis sampler and a BioSampler for collecting four different types of bioaerosols, including Escherichia coli, Staphylococcus aureus, Candida famata and endospores of Bacillus subtilis, were compared in a chamber study. The results showed that the ATP assay may indicate the four microbes' viability, and that their defined viabilities were positively correlated with their culturability. In addition, the optimal sampling conditions of the

SUBMITTER: Tseng CC 

PROVIDER: S-EPMC7409044 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature