Isolation and Characterization of Extracellular Vesicles Secreted In Vitro by Porcine Microbiota.

ABSTRACT: The secretion of extracellular vesicles, EVs, is a common process in both prokaryotic and eukaryotic cells for intercellular communication, survival, and pathogenesis. Previous studies have illustrated the presence of EVs in supernatants from pure cultures of bacteria, including Gram-positive and Gram-negative glycan-degrading gut commensals. However, the isolation and characterization of EVs secreted by a complex microbial community have not been clearly reported. In a recent paper, we showed that wood-derived, complex β-mannan, which shares a structural similarity with conventional dietary fibers, can be used to modulate the porcine gut microbiota composition and activity. In this paper, we investigated the production, size, composition, and proteome of EVs secreted by pig fecal microbio

SUBMITTER: Lagos L 

PROVIDER: S-EPMC7409281 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature