Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

ABSTRACT:

Background

HIV-1 infects a wide range of CD4⁺ T cells with different phenotypic properties and differing expression levels of entry coreceptors. We sought to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4⁺ T cell subsets and whether tropism changes during acute to chronic disease progression. HIV-1 envs were amplified from the plasma of five C-HIV infected women from three untreated time points; less than 2 months, 1-year and 3-years post-infection. Pseudoviruses were generated from Env clones, phenotyped for coreceptor usage and CD4⁺ T cell subset tropism was measured by flow cytometry.

Results

A total of 50 C-HIV envs were cloned and screened for functionality in pseudovirus infection assays. Phylog

SUBMITTER: Gartner MJ 

PROVIDER: S-EPMC7409430 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature