The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission in transgenic chickens.

ABSTRACT:

Background

The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G₂ transgenic chickens to evaluate suppression of viral transmission.

Result

The transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p < 0.05) in mean death time after being directly chal

SUBMITTER: Byun SJ 

PROVIDER: S-EPMC7409462 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature