Rapid and durable photochemical bonding of cartilage using the porphyrin photosensitizer verteporfin.

ABSTRACT:

Objective

To evaluate the effectiveness of verteporfin as a photosensitizer to photochemically bond articular cartilage tissues and determine bond durability in vitro.

Design

Bond strength induced by verteporfin over a range of concentrations and light exposure conditions was investigated using a disk-annulus model and a pushout test. Exposure was parameterized by varying either irradiance or irradiation time. Bond robustness in a cell-mediated degeneration environment was examined by exposing newly bonded samples to interleukin-1 alpha for the first 4 days of a 7-day culture period, followed by mechanical testing and biochemical and cellular viability assays.

Results

Photochemical bonding using verteporfin produced high bonding shear strengths at relatively low photo

SUBMITTER: Arvayo AL 

PROVIDER: S-EPMC7409723 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature