Reference Gene Optimization for Circadian Gene Expression Analysis in Human Adipose Tissue.

ABSTRACT: A hallmark of biology is the cyclical nature of organismal physiology driven by networks of biological, including circadian, rhythms. Unsurprisingly, disruptions of the circadian rhythms through sleep curtailment or shift work have been connected through numerous studies to positive associations with obesity, insulin resistance, and diabetes. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) measures oscillation in messenger RNA expression, an essential foundation for the study of the physiological circadian regulatory network. Primarily, measured oscillations have involved the use of reference gene normalization. However, the validation and identification of suitable reference genes is a significant challenge across different biological systems. This study focuses on

SUBMITTER: White JM 

PROVIDER: S-EPMC7409766 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature