Cystic fibrosis gene mutations and polymorphisms in Saudi men with infertility.

ABSTRACT:

Background

Some mutations of the cystic fibrosis transmembrane regulator (CFTR) gene may impair spermatogenesis or cause a congenital absence of the vas deferens that manifests as isolated male infertility.

Objective

Assess the frequency and analyze the spectrum of CFTR gene variations in Saudi men with primary infertility.

Design

Prospective, cross-sectional.

Setting

Tertiary care specialist hospital in Jeddah.

Patients and methods

Genomic DNA was extracted from peripheral blood samples of Saudi men who presented with primary infertility to the outpatient andrology clinic with either azoospermia or oligoasthenoteratozoospermia. Polymerase chain reaction and direct sequencing were used to identify all variants of the CFTR gene.

Ma

SUBMITTER: AlMaghamsi T 

PROVIDER: S-EPMC7410224 | biostudies-literature | 2020 Jul-Aug

REPOSITORIES: biostudies-literature