The changing mouse embryo transcriptome at whole tissue and single-cell resolution.

ABSTRACT: During mammalian embryogenesis, differential gene expression gradually builds the identity and complexity of each tissue and organ system¹. Here we systematically quantified mouse polyA-RNA from day 10.5 of embryonic development to birth, sampling 17 tissues and organs. The resulting developmental transcriptome is globally structured by dynamic cytodifferentiation, body-axis and cell-proliferation gene sets that were further characterized by the transcription factor motif codes of their promoters. We decomposed the tissue-level transcriptome using single-cell RNA-seq (sequencing of RNA reverse transcribed into cDNA) and found that neurogenesis and haematopoiesis dominate at both the gene and cellular levels, jointly accounting for one-third of differential gene expression and mo

SUBMITTER: He P 

PROVIDER: S-EPMC7410830 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature