[¹⁸F]FEPPA PET imaging for monitoring CD68-positive microglia/macrophage neuroinflammation in nonhuman primates.

ABSTRACT:

Purpose

The aim of this study was to examine whether the translocator protein 18-kDa (TSPO) PET ligand [¹⁸F]FEPPA has the sensitivity for detecting changes in CD68-positive microglial/macrophage activation in hemiparkinsonian rhesus macaques treated with allogeneic grafts of induced pluripotent stem cell-derived midbrain dopaminergic neurons (iPSC-mDA).

Methods

In vivo positron emission tomography (PET) imaging with [¹⁸F]FEPPA was used in conjunction with postmortem CD68 immunostaining to evaluate neuroinflammation in the brains of hemiparkinsonian rhesus macaques (n = 6) that received allogeneic iPSC-mDA grafts in the putamen ipsilateral to MPTP administration.

Results

Based on assessment of radiotracer uptake and confirmed by visual inspection

SUBMITTER: Zammit M 

PROVIDER: S-EPMC7410886 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature