A Four-Hour Carbapenem Inactivation Method (CIM ^B.S ) Using Bacillus stearothermophilus as Indicator Strain.

ABSTRACT: Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here we used Bacillus stearothermophilus as an indicator strain in the format of the carbapenem inactivation method (CIM) procedure to develop a rapid carbapenemase phenotype detection method: CIM^B.S. Methods: The CIM^B.S test was derived from the mCIM, where B. stearothermophilus replaced Escherichia coli as the indicator strain. The test bacteria were incubated in the presence of imipenem for 30 min, and then, aliquots were placed on colorimetric plates, and incubation was continued for 3.5 h at 60°C. We examined 134 clinical strains to evaluate the CIM^B.S performance. Results: The CIM^B.S

SUBMITTER: Cui ZH 

PROVIDER: S-EPMC7411124 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature