Expression of Notch family is altered in non‑alcoholic fatty liver disease.

ABSTRACT: The aim of the present study was to explore the dynamic relationship between Notch and non‑alcoholic fatty liver disease (NAFLD), both in vitro and in vivo. The LX2, Huh7 and MIHA hepatic cell lines were used to establish a cell steatosis model induced by palmitic acid (PA) at different concentrations (0.1, 0.25 and 0.5 mM). Cell proliferation and migration were assessed using a 5‑bromo‑2'‑deoxyuridine kit and a wound healing assay. The dosage of 0.25 mM PA for 36‑48 h treatment was chosen for subsequent experiments. Steatotic cells were identified by Oil Red O staining. Feeding mice a methionine‑choline‑deficient (MCD) diet is known induce a model of NAFLD, compared with a methionine‑choline‑sufficient (MCS) diet. Therefore, Notch family mRNA expression was evaluated in the liver of MCD‑f

SUBMITTER: Ding WJ 

PROVIDER: S-EPMC7411296 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature