Poison ivy hairy root cultures enable a stable transformation system suitable for detailed investigation of urushiol metabolism.

ABSTRACT: Poison ivy (Toxicodendron radicans) is best known for causing exasperating allergenic delayed-contact dermatitis symptoms that last for weeks on persons who have contacted the plant. Urushiols are alkylcatechols produced by poison ivy responsible for causing this dermatitis. While urushiol chemical structures are well known, the metabolic intermediates and genes responsible for their biosynthesis have not been experimentally validated. A molecular genetic characterization of urushiol biosynthesis in poison ivy will require stable genetic transformation and subsequent regeneration of organs that retain the capacity synthesize urushiol. To this end, Agrobacterium rhizogenes was used to generate hormone-independent poison ivy hairy root cultures. Optimal conditions for hairy roo

SUBMITTER: Lott AA 

PROVIDER: S-EPMC7411346 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature