Scorpion primer PCR analysis for genotyping of allele variants of thiopurine s‑methyltransferase*3.

ABSTRACT: Thiopurine S-methyltransferase (TPMT) plays an important role in the metabolism of thiopurines. Mutations in the TPMT gene can affect drug activity, which may have adverse effects in humans. Thus, genotyping can help elucidate genetic determinants of drug response to thiopurines and optimize the selection of drug therapies for individual patients, effectively avoiding palindromia during maintenance treatment caused by insufficient dosing and the serious side effects caused by excessive doses. The current available detection methods used for TPMT*3B and TPMT*3C are complex, costly and time‑consuming. Therefore, innovative detection methods for TPMT genotyping are urgently required. The aim of the present study was to establish and optimize a simple, specific and timesaving TPMT genotyping m

SUBMITTER: Yao P 

PROVIDER: S-EPMC7411393 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature