Xanthine Oxidase Inhibition and Anti-LDL Oxidation by Prenylated Isoflavones from Flemingia philippinensis Root.

ABSTRACT: Xanthine oxidase is a frontier enzyme to produce oxidants, which leads to inflammation in the blood. Prenylated isoflavones from Flemingia philippinensis were found to display potent inhibition against xanthine oxidase (XO). All isolates (1-9) inhibited XO enzyme with IC₅₀ ranging 7.8~36.4 μM. The most active isoflavones (2-5, IC₅₀ = 7.8~14.8 μM) have the structural feature of a catechol motif in B-ring. Inhibitory behaviors were disclosed as a mixed type I mode of inhibition with K_I < K_IS. Binding affinities to XO enzyme were evaluated. Fluorescence quenching effects agreed with inhibitory potencies (IC₅₀s). The compounds (2-5) also showed potent anti-LDL oxidation effects

SUBMITTER: Kim JY 

PROVIDER: S-EPMC7411605 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature