PI 3-kinase delta enhances axonal PIP₃ to support axon regeneration in the adult CNS.

ABSTRACT: Peripheral nervous system (PNS) neurons support axon regeneration into adulthood, whereas central nervous system (CNS) neurons lose regenerative ability after development. To better understand this decline whilst aiming to improve regeneration, we focused on phosphoinositide 3-kinase (PI3K) and its product phosphatidylinositol (3,4,5)-trisphosphate (PIP₃ ). We demonstrate that adult PNS neurons utilise two catalytic subunits of PI3K for axon regeneration: p110α and p110δ. However, in the CNS, axonal PIP₃ decreases with development at the time when axon transport declines and regenerative competence is lost. Overexpressing p110α in CNS neurons had no effect; however, expression of p110δ restored axonal PIP₃ and increased regenerative axon transport. p110δ ex

SUBMITTER: Nieuwenhuis B 

PROVIDER: S-EPMC7411663 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature