Assessing Genome-Wide Diversity in European Hantaviruses through Sequence Capture from Natural Host Samples.

ABSTRACT: Research on the ecology and evolution of viruses is often hampered by the limitation of sequence information to short parts of the genomes or single genomes derived from cultures. In this study, we use hybrid sequence capture enrichment in combination with high-throughput sequencing to provide efficient access to full genomes of European hantaviruses from rodent samples obtained in the field. We applied this methodology to Tula (TULV) and Puumala (PUUV) orthohantaviruses for which analyses from natural host samples are typically restricted to partial sequences of their tri-segmented RNA genome. We assembled a total of ten novel hantavirus genomes de novo with very high coverage (on average >99%) and sequencing depth (average >247×). A comparison with partial Sanger sequences indicat

SUBMITTER: Hiltbrunner M 

PROVIDER: S-EPMC7412162 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature