TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection.

ABSTRACT:

Background

Toxoplasma gondii is an opportunistic protozoan infecting almost one-third of the world's population. Toxoplasma gondii rhoptry protein 18 (TgROP18) is a key virulence factor determining the parasite's acute virulence and is secreted into host cells during infection. We previously identified the interaction of TgROP18 and host cell immune-related receptor protein IL20RB, and observed the activation of STAT3 in human keratinocytes (HaCaT) cells infected by the rop16 knockout RH strain, though TgROP16 is regarded as being responsible for host STAT3 activation during T. gondii invasion. Therefore, we hypothesize TgROP18 can activate host STAT3 through binding to IL20RB.

Methods

CRISPR-CAS9 technology was used to generate the ROP16 and ROP18 double knockout RH strain,

SUBMITTER: Kong L 

PROVIDER: S-EPMC7412674 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature