Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients.

ABSTRACT:

Background

The aetiologies and pathogeneses of the joint diseases rheumatoid arthritis (RA) and spondyloarthritis (SpA) are still not fully elucidated. To increase our understanding of the molecular pathogenesis, we analysed the protein composition of synovial fluid (SF) from rheumatoid arthritis (RA) and spondyloarthritis (SpA) patients.

Methods

Fifty-six synovial fluid samples (RA, n = 32; SpA, n = 24) were digested with trypsin, and the resulting peptides were separated by liquid chromatography and analysed by tandem mass spectrometry. Additionally, the concentration of cell-free DNA (cfDNA) in the synovial fluid was measured, and plasma C-reactive protein (CRP) was determined.

Results

Three hundred thirty five proteins were identified within the SF. The more abund

SUBMITTER: Birkelund S 

PROVIDER: S-EPMC7412817 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature