Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.

ABSTRACT: Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste material produced by heterologous overexpression of recombinant genes. In industrial applications, they are occasionally used as an alternative in cases where a protein cannot be expressed in soluble form and in high enough amounts. Then, however, refolding approaches are needed to transform inactive IBs into active soluble protein. While anecdotal reports about IBs themselves showing catalytic functionality/activity (CatIB) are found throughout literature, only recently, the use of protein engineering methods has facilitated the on-demand production of CatIBs. CatIB formation is induced usually by fusing short peptide tags or aggregation-inducing protein domains to a target protein. The resulting protei

SUBMITTER: Jager VD 

PROVIDER: S-EPMC7413871 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature