Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production.

ABSTRACT: Rhodothermus marinus has the potential to be well suited for biorefineries, as an aerobic thermophile that produces thermostable enzymes and is able to utilize polysaccharides from different 2nd and 3rd generation biomass. The bacterium produces valuable chemicals such as carotenoids. However, the native carotenoids are not established for industrial production and R. marinus needs to be genetically modified to produce higher value carotenoids. Here we genetically modified the carotenoid biosynthetic gene cluster resulting in three different mutants, most importantly the lycopene producing mutant TK-3 (ΔtrpBΔpurAΔcruFcrtB::trpBcrtB _{T.thermophilus} ). The genetic modifications and subsequent structural analysis of carotenoids helped clarif

SUBMITTER: Kristjansdottir T 

PROVIDER: S-EPMC7414004 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature