Retrograde cannulation of femoral artery: A novel experimental design for precise elicitation of vasosensory reflexes in anesthetized rats.

ABSTRACT: Vasosensory reflex responses are elicited by instillation of nociceptive agents in a segment of peripheral blood vessel. A novel method for the stimulation of perivascular afferents was designed by retrograde cannulation of femoral artery, using a 24G, double ported polyethylene cannula. The vertical port of which was used to inject the algogen into the artery and horizontal port to measure the BP continuously, as this port was connected to the pressure transducer. Previously, separate carotid artery cannulation was used for the BP recording. But our experimental design excluded the need for carotid artery cannulation that might compromise the circulation to the CNS centers mediating cardiorespiratory reflex responses. After cannulation, the proximal end of femoral artery became an end art

SUBMITTER: Revand R 

PROVIDER: S-EPMC7414010 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature