Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster.

ABSTRACT: In recent years, the gasotransmitter hydrogen sulphide (H₂S), produced by the transsulphuration pathway, has been recognized as a biological mediator playing an important role under normal conditions and in various pathologies in both eukaryotes and prokaryotes. The transsulphuration pathway (TSP) includes the conversion of homocysteine to cysteine following the breakdown of methionine. In Drosophila melanogaster and other eukaryotes, H₂S is produced by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulphurtransferase (MST). In the experiments performed in this study, we were able to explore the CRISPR/Cas9 technique to obtain single and double deletions in homozygotes of these three major genes responsible for H₂S produ

SUBMITTER: Zatsepina O 

PROVIDER: S-EPMC7414014 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature