The gain-of-function allele bamA_E470K bypasses the essential requirement for BamD in β-barrel outer membrane protein assembly.

ABSTRACT: The outer membrane (OM) of gram-negative bacteria confers innate resistance to toxins and antibiotics. Integral β-barrel outer membrane proteins (OMPs) function to establish and maintain the selective permeability of the OM. OMPs are assembled into the OM by the β-barrel assembly machine (BAM), which is composed of one OMP-BamA-and four lipoproteins-BamB, C, D, and E. BamB, C, and E can be removed individually with only minor effects on barrier function; however, depletion of either BamA or BamD causes a global defect in OMP assembly and results in cell death. We have identified a gain-of-function mutation, bamA_E470K , that bypasses the requirement for BamD. Although bamD::kan bamA_E470K cells exhibit growth and OM barrier defects, th

SUBMITTER: Hart EM 

PROVIDER: S-EPMC7414184 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature