Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening.

ABSTRACT: Baculovirus mediated-insect cell expression systems have been widely used for producing heterogeneous proteins. However, to date, there is still the lack of an easy-to-manipulate system that enables the high-throughput protein characterization in insect cells by taking advantage of large existing Gateway clone libraries. To resolve this limitation, we have constructed a suite of Gateway-compatible pIEx-derived baculovirus expression vectors that allow the rapid and cost-effective construction of expression clones for mass parallel protein expression in insect cells. This vector collection also supports the attachment of a variety of fusion tags to target proteins to meet the needs for different research applications. We first demonstrated the utility of these vectors for protein expression

SUBMITTER: Tang Y 

PROVIDER: S-EPMC7414197 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature