Structure-based evolution of a promiscuous inhibitor to a selective stabilizer of protein-protein interactions.

ABSTRACT: The systematic stabilization of protein-protein interactions (PPI) has great potential as innovative drug discovery strategy to target novel and hard-to-drug protein classes. The current lack of chemical starting points and focused screening opportunities limits the identification of small molecule stabilizers that engage two proteins simultaneously. Starting from our previously described virtual screening strategy to identify inhibitors of 14-3-3 proteins, we report a conceptual molecular docking approach providing concrete entries for discovery and rational optimization of stabilizers for the interaction of 14-3-3 with the carbohydrate-response element-binding protein (ChREBP). X-ray crystallography reveals a distinct difference in the binding modes between weak and general inhibitors of

SUBMITTER: Sijbesma E 

PROVIDER: S-EPMC7414219 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature