Characterization of Uveal Melanoma Cell Lines and Primary Tumor Samples in 3D Culture.

ABSTRACT:

Purpose

Uveal melanoma (UM) typically spreads to the liver, where it is incurable, as there are limited therapeutic interventions available. This study aimed to standardize laboratory methods for generating three-dimensional (3D) spheroids using UM cell lines and primary UM (PUM) samples for use in drug screening.

Methods

Six UM cell lines and nine PUM, of differing genetic characteristics were cultured in two dimensions (2D) and three dimensions. 3D spheroid formation and growth were time monitored, and ImageJ software was used to calculate cross-sectional areas. PUM spheroids underwent immunohistochemistry for melanoma markers, nuclear BAP1, and cell proliferation. Chromosomal alterations in patient UM biopsies were compared with the corresponding 3D spheroid. In vitro dru

SUBMITTER: Aughton K 

PROVIDER: S-EPMC7414609 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature