Localized Photoreceptor Ablation Using Femtosecond Pulses Focused With Adaptive Optics.

ABSTRACT:

Purpose

The development of new approaches to human vision restoration could be greatly accelerated with the use of nonhuman primate models; however, there is a paucity of primate models of outer retina degeneration with good spatial localization. To limit ablation to the photoreceptors, we developed a new approach that uses a near-infrared ultrafast laser, focused using adaptive optics, to concentrate light in a small focal volume within the retina.

Methods

In the eyes of eight anesthetized macaques, 187 locations were exposed to laser powers from 50 to 210 mW. Laser exposure locations were monitored for up to 18 months using fluorescein angiography (FA), optical coherence tomography (OCT), scanning laser ophthalmoscopy (SLO), adaptive optics scanning laser ophthalmoscope (A

SUBMITTER: Dhakal KR 

PROVIDER: S-EPMC7414617 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature