Differential chloride homeostasis in the spinal dorsal horn locally shapes synaptic metaplasticity and modality-specific sensitization.

ABSTRACT: GABA_A/glycine-mediated neuronal inhibition critically depends on intracellular chloride (Cl^-) concentration which is mainly regulated by the K⁺-Cl^- co-transporter 2 (KCC2) in the adult central nervous system (CNS). KCC2 heterogeneity thus affects information processing across CNS areas. Here, we uncover a gradient in Cl^- extrusion capacity across the superficial dorsal horn (SDH) of the spinal cord (laminae I-II: LI-LII), which remains concealed under low Cl^- load. Under high Cl^- load or heightened synaptic drive, lower Cl^- extrusion is unveiled in LI, as expected from the gradient in KCC2 expression found across the SDH. Blocking TrkB receptors increases KCC2 in LI, pointing to differential constitutiv

SUBMITTER: Ferrini F 

PROVIDER: S-EPMC7414850 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature