Transcriptional Organization of the Stability Module of Broad-Host-Range Plasmid RA3, from the IncU Group.

ABSTRACT: The broad-host-range (BHR) conjugative plasmids have developed diverse adaptive mechanisms defining the range of their promiscuity. The BHR conjugative RA3 plasmid, the archetype of the IncU group, can transfer between, replicate in, and be maintained in representatives of Alpha-, Beta-, and Gammaproteobacteria Its stability module encompasses ten open reading frames (ORFs) apparently organized into five operons, all transcribed in the same direction from several strong promoters that are tightly regulated either by autorepressors or by global plasmid-encoded regulators. In this paper, we demonstrate that owing to an efficient RNA polymerase (RNAP) read-through, the transcription from the first promoter, orf02p, may continue through the whole module. Moreover, a

SUBMITTER: Lewicka E 

PROVIDER: S-EPMC7414963 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature