Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury.

ABSTRACT:

Introduction

Astrogliosis and glial scar formation following spinal cord injury (SCI) are viewed as major obstacles that hinder axonal regeneration and functional recovery. Regulating the glial scar and axonal regeneration in the lesion site is important for treating SCI.

Aims

Considering the important role of astrocyte in glial scar formation and subsequent axonal regeneration, we intended to investigate the effect of the transcription factors OCT4 and KLF4 on astrocyte and the underlying mechanism after spinal cord contusion injury in transgenic mice.

Results

Western blotting, q-PCR, immunofluorescence, and functional evaluation suggested that glial fibrillary acidic protein (GFAP) expression decreased in the lesion area, the porosity of the scar increased, and remy

SUBMITTER: Huang X 

PROVIDER: S-EPMC7415207 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature